ASSESSMENT OF THE EFFECT OF CONNECTIVE TISSUE CELLS ON THE REGENERATION OF THE ORAL MUCOSA

Authors

  • Khusanova Yu.B., Khramova N.V. Tashkent State Dental Institute (Tashkent, Uzbekistan)
  • Charyshnikova O.S., Tsiferova N.A. Center for Advanced Technologies (Tashkent, Uzbekistan)

Keywords:

tissue-engineered construct, allofibroblasts, silk degummed gauze, experimental animals.

Abstract

This study presents a comparative experimental analysis of a tissue-engineered construct composed of silk degummed gauze and allofibroblasts for the treatment of mucosal defects in rats. The dynamics of animal weight and the relative weight of internal organs did not exhibit statistically significant deviations compared to control and reference values, indicating the safety of the cell therapy and tissue engineering methods employed for the experimental animals. The proposed tissue-engineered construct effectively supports the maintenance of proliferating dermal fibroblasts within the scaffold.

References

Ahmed S., Chauhan V. M., Ghaemmaghami A. M., Aylott J. W. (2019). New Generation of Bioreactors that advance Extracellular Matrix Modelling and Tissue Engineering. Biotechnol. Lett. 41 (1), 1-25. 10.1007/s10529-018-2611-7 - DOI - PMC

Anderson L., Karring T., Mackenzie I. Oral mucous membrane. Human Oral Embryology and Histology. Copenhagen: Munksgaard, Eds.I.A. Mjor, O. Fejerskov,1986. P.203-242.

Goldenberg D, McLaughlin C, Koduru SV, Ravnic DJ. Regenerative Engineering: Current Applications and Future Perspectives. Front Surg. 2021 Nov 3;8:731031. doi: 10.3389/fsurg.2021.731031 eCollection 2021.

Jadbabaei S, Kolahdoozan M, Naeimi F, Ebadi-Dehaghani H. Preparation and characterization of sodium alginate-PVA polymeric scaffolds by electrospinning method for skin tissue engineering applications. RSC Adv. 2021 Sep 15;11(49):30674-30688. doi: 10.1039/d1ra04176b. eCollection 2021 Sep 14.

Khramova N.V. Xusanova Y. B., Makhmudov A. A. Regarding the regeneration of superficial skin defects. Central Asian Journal of Medicine. Tashkent, 2021; 2: 115-118.

Li Xiao 1, Yan Du, Yang Shen, Ying He, Hui Zhao, Zhenhua Li. TGF-beta 1 induced fibroblast proliferation is mediated by the FGF-2/ERK pathway. Front Biosci (Landmark Ed). 2012 Jun 1;17(7):2667-74. doi: 10.2741/4077

Luangbudnark W, Viyoch J, Laupattarakasem W, Surakunprapha P, Laupattarakasem P. Properties and Biocompatibility of Chitosan and Silk Fibroin Blend Films for Application in Skin Tissue Engineering. The Sci World J. 2012; 2012: 1-10, doi: 10.1100/2012/697201.

Moretti L, Stalfort J, Barker TK, Abebayehu D. J The interplay of fibroblasts, the extracellular matrix, and inflammation in scar formation. Biol Chem. 2022 Feb;298(2):101530. doi: 10.1016/j.jbc.2021.101530. Epub 2021 Dec 23.

Neishabouri A, Soltani Khaboushan A, Daghigh F, Kajbafzadeh AM, Majidi Zolbin M. Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods. Front Bioeng Biotechnol. 2022 Apr 25; 10:805299. doi: 10.3389/fbioe.2022.805299. eCollection 2022.

Pitaru S., Tal K., Soldinger M. et al. Collagen membranes prevent the apical migration of epithelium during periodontal wound healing. J. Periodont. Res. 1987; 22: 331-333.

Downloads

Published

2025-06-07

How to Cite

Khusanova Yu.B., Khramova N.V., & Charyshnikova O.S., Tsiferova N.A. (2025). ASSESSMENT OF THE EFFECT OF CONNECTIVE TISSUE CELLS ON THE REGENERATION OF THE ORAL MUCOSA. Central Asian Journal of Medicine, (4), 193 - 195. Retrieved from https://journals.tma.uz/index.php/cajm/article/view/1391

Issue

Section

Articles