CELLULAR IMMUNE RESPONSE IN EXPERIMENTAL MODELING OF COMPLICATED AND UNCOMPLICATED HYDATIDOSIS ECHINOCOCCOSIS OF THE LIVER
Keywords:
Hydatidosis echinococcosis of the liver, cellular immunity, peripheral blood mononuclear cellsAbstract
Background. The incidence of echinococcosis of internal organs, due to the peculiarities of the pathomorphological manifestations of the disease, in the form of the organisation of specific cysts prone to dissemination, is considered very costly for the social sphere. Given that this disease most often affects people of working age, the pathology is also reflected in an increase in the number of disabilities, and the absence of treatment, in an increase in mortality.
Material and methods. The basis of the experimental material was made up of rabbits of the Chinchilla breed. The expression of CD-differentiated and activation antigens assessed the quantitative determination of cellular immunity. The following markers of immunocompetent cells were determined: CD4+CD28+, CD8+CD28+, CD4+CD25+. CD receptor expression was performed in a rosette formation reaction using LT series monoclonal antibodies manufactured by Sorbent LLC (Russia).
Results. Absolute and significant elevation of CD4+CD28+ was noted at all stages of modelling of hydatidosis echinococcosis of the liver (p<0.05). An increase in the number of CD4+CD25+ was noted in the late stages of the modelling of the pathological process. At the same time, against this background, there was a decrease in the number of CD8+CD28+, which indicates differentiated changes in the mononuclear cells of peripheral blood themselves.
Conclusion. Reproduction of the experimental model of hydatidosis echinococcosis of the liver leads to differentiated expression of lymphocyte subpopulations, transforming the body's defence response into a kind of immune response, which was characterized by the activity of T-cells to organize the process and form a protective layer. However, this response of the organism under the influence of a secondary, non-specific bacterial infection changed its nature of the immune trace, which can be traced in the following analysis.
