DEVELOPMENT AND COMPARATIVE EVALUATION OF THE EFFECTIVENESS OF A NEW EXPERIMENTAL MODEL OF ABDOMINAL SEPSIS
DOI:
https://doi.org/10.61934/jesm.v1i1.670Keywords:
Abdominal sepsis, reproduction of the experimental model, peritonitisAbstract
Background. The main goal of modelling pathological processes is to solve the problem of an entire link of translational medicine, which is aimed at developing treatment methods and introducing them into clinical settings.
Material and methods. The work is experimental and was carried out on 40 beads of both sexes of the Wistar line. Various variants of abdominal sepsis were simulated in animals, including the original technique developed by us.
Results. Comparative characteristics of the reproducibility of various variants of experimental models of abdominal sepsis showed high values of positive results among animals of the C-series. The reproducibility rate of abdominal sepsis was 28.5% higher than among Series-A and Series-B animals. At the same time, lethality among the animals of the C-series was minimal.
Conclusion. The proposed version of the model of abdominal sepsis makes it possible to achieve the formation of several pathogenetic mechanisms that have a reasonable place in clinical practice.
References
A review. Lessons from an animal model of intra-abdominal sepsis / J. G. Bartlett, A. B. Onderdonk, T. Louie, D. L. Kasper, and S. L. Gorbach // Archives of Surgery, 2018, vol. 113, # 7, pp. 853–857.
An improved clinically relevant sepsis model in the conscious rat / G. Mathiak, D. Szewczyk, F. Abdullah, P. Ovadia, G. Feuerstein, and R. Rabinovici // Critical Care Medicine, 2020, vol. 28, # 6, pp. 1947–1952.
Angus DC, van der Poll T: Severe sepsis and septic shock. N Engl J Med 2013;369:840–851.
Animal model for chronic-abscess-forming peritonitis: histology and microbiology / H. W. Volk, J. Schneider, J. Dämmrich, W. Döll, M. Hörl, and H. P. Bruch // European Surgical Research, 2020, vol. 22, # 6, pp. 347–355.
Azizov Y.H., Okhunov A.O., Azizova, P.H. Metabolic activity of lungs in the development of an experimental model of surgical sepsis. // European Science Review, 2018, V.11, #12, P.66-69.
Babadzhanov B.D., Fayziev Sh.D., Okhunov A.O., Atakov S.S. Method of modeling acute purulent-destructive lung disease // Patent for invention No. 192 PV RUz dated 09.11.1992.
Biomarkers of sepsis / J. C. Marshall, K. Reinhart, and International Sepsis Forum // Critical Care Medicine, 2019, vol. 37, # 7, pp. 2290–2298.
Boivin, In Bacteries et Virus, Presses Universitaires de France, Paris, 1941.
Boldingh Q, de Vries F, Boermeester MA: Abdominal Sepsis. Curr Opin Crit Care 2017;23(2):159–166.
Borden W., Hall W. H., Fatal transfusion reactions from massive bacterial contamination of blood // New England Journal of Medicine, 1951, vol. 245, # 20, pp. 760–765.
Browne M. K., Leslie G. B. Animal models of peritonitis // Surgery, gynecology & obstetrics, 1976, vol. 143, pp. 738–740.
Cecal ligation and incision: an acute onset model of severe sepsis in rats / P. Scheiermann, S. Hoegl, M. Revermann et al. // The Journal of Surgical Research, 2009, vol. 151, # 1, pp. 132–137.
Cecal ligation and puncture / W. J. Hubbard, M. Choudhry, M. G. Schwacha et al. // Shock, 2015, vol. 24, Supplement 1, pp. 52–57.
Cecal ligation and puncture versus colon ascendens stent peritonitis: two distinct animal models for polymicrobial sepsis / S. Maier, T. Traeger, M. Entleutner et al. // Shock, 2024, vol. 21, # 6, pp. 505–512.
Cecum ligation and dissection: a novel modified mouse sepsis model / H. Mutlak, C. Jennewein, N. Tran et al. // The Journal of Surgical Research, 2013, vol. 183, # 1, pp. 321–329.
Characteristics of clinical sepsis reflected in a reliable and reproducible rodent sepsis model / F. A. Gonnert, P. Recknagel, M. Seidel et al. // The Journal of Surgical Research, 2011, vol. 170, # 1, pp. e123–e134.
Chichom-Mefire A, Fon TA, Ngowe-Ngowe M: Which cause of diffuse peritonitis is the deadliest in the tropics? A retrospective analysis of 305 cases from the southwest region of Cameroon. World J Emerg Surg 2016;11:14.
Choice of bacteria in animal models of sepsis / S. Cross, S. M. Opal, J. C. Sadoff, and P. Gemski // Infection and Immunity, 2023, vol. 61, # 7, pp. 2741–2747.
Dose-dependent mortality and organ injury in a cecal slurry peritonitis model / M. J. Lee, K. Kim, Y. H. Jo, J. H. Lee, and J. E. Hwang // The Journal of Surgical Research, 2016, vol. 206, # 2, pp. 427–434.
Early events after intra-abdominal infection with Bacteroides fragilis and Escherichia coli / W. R. Verweij, F. Namavar, W. F. Schouten, and D. M. MacLaren // Journal of Medical Microbiology, 1991, vol. 35, # 1, pp. 18–22.
Essential role of gamma interferon in the survival of colon ascendens stent peritonitis, a novel murine model of abdominal sepsis / N. Zantl, A. Uebe, B. Neumann et al. // Infection and immunity, 1998, vol. 66, no. 5, pp. 2300–2309.
Hotchkiss R. S., Karl I. E. The pathophysiology and treatment of sepsis // The New England Journal of Medicine, 2023, vol. 348, # 2, pp. 138–150.
Kirkpatrick AW, Hamilton DR, McKee JL, et al: Do we have the guts to go? The abdominal compartment, intra-abdominal hypertension, the human microbiome, and exploration class space missions Can J Surg 2020;63:E581–E593.
Lozano R, Naghavi M, Foreman K, et al: Global and regional mortality from 235 causes of death for 20 age groups in1990 and 2010: A systematic analysis for the global burden of disease study 2010. Lancet 2012;380(9859):2095–2128.
Massive chemokine transcription in acute renal failure due to polymicrobial sepsis / S. Maier, K. Emmanuilidis, M. Entleutner et al. // Shock, 2020, vol. 14, # 2, pp. 187–192.
Microbial synergy in experimental intra-abdominal abscess / B. Onderdonk, J. G. Bartlett, T. Louie, N. Sullivan-Seigler, and S. L. Gorbach // Infection and Immunity, 1976, vol. 13, # 1, pp. 22–26.
Murray CJL, Vos T, Lozano R, et al: Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 Regions, 1990-2010: A systematic analysis for the global burden of disease study 2010. Lancet 2012;380(9859):2197–2223.
Okhunov A.O., Pulatov U.I., Okhunova D.A. Clinical and laboratory characteristics of the course of the wound process of soft tissues. Bulletin of Science and Education, 2018, V.9, #45, p. 104-110.
Overwhelming bacteremic shock produced by gram-negative bacilli: a report of four cases with one recovery / E. Braude, J. Siemienski, D. Williams, J. P. Sanford // University of Michigan Medical Bulletin, 1953, vol. 19, pp. 23–42.
Parker S. J., Watkins P. E. Experimental models of gram-negative sepsis // The British Journal of Surgery, 2001, vol. 88, # 1, pp. 22–30.
Resilience to bacterial infection: the difference between species could be due to proteins in serum / H. S. Warren, C. Fitting, E. Hoff et al. // The Journal of Infectious Diseases, 2020, vol. 201, # 2, pp. 223–232.
Rittirsch D., Hoesel L. M., Ward P. A. The disconnect between animal models of sepsis and human sepsis // Journal of Leukocyte Biology, 2017, vol. 81, # 1, pp. 137–143.
Sartelli Catena F, Ansaloni L, Coccolini F, et al: Complicated intra-abdominal infections worldwide: The definitive data of the CIAOW study. World J Emerg Surg 2014;9:37.
Sepsis and septic shock--A review of laboratory models and a proposal / S. Keith, K. A. Wichterman, A. E. Baue, and I. H. Chaudry // Journal of Surgical Research, 1980, vol. 29, # 2, pp. 189–201.
Singleton K. D., Wischmeyer P. E. Distance of cecum ligated influences mortality, tumor necrosis factor-alpha and interleukin-6 expression following cecal ligation and puncture in the rat // European Surgical Research, 2023, vol. 35, # 6, pp. 486–491.
Specific changes in lactoferrin content in various blood samples in an experimental model of acute respiratory distress syndrome of extrapulmonary genesis. A.O. Okhunov, O.T. Sattarov, E.Kh. Azizov, I.A. Ziyakulov, B.O. Sakhobov // Doctor-Graduate Student, 2011, 46(3.3), pp.445-449.
Stewart B, Khanduri P, McCord C, et al: Global disease burden of conditions requiring emergency surgery. BJS 2014;101:e9–e22.
Tellor B, Skrupky LP, Symons W, et al: Inadequate source control and inappropriate antibiotics are key determinants of mortality in patients with intra-abdominal sepsis and associated bacteremia. Surg Infect 2015;16(6):785–793.
Weber G. F., Swirski F. K. Immunopathogenesis of abdominal sepsis, // Langenbeck's Archives of Surgery, 2023, vol. 399, # 1, pp. 1–9.
